Categories: 8th Semester

GTU BE 8th Semester 2180408 Biochemical Engineering-II Summer 2018 Question Paper

1
Seat No.: ________ Enrolment No.___________
GUJARAT TECHNOLOGICAL UNIVERSITY
BE – SEMESTER ?VIII (NEW) – EXAMINATION ? SUMMER 2018
Subject Code: 2180408 Date: 30/04/2018
Subject Name: Biochemical Engineering-II
Time: 10:30 AM to 01:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
4. Notations / abbreviations have conventional meaning and needs no clarification.

MARKS
Q.1 (a) What are the objectives of downstream processes?
03
(b) What are the categories of microbial product that can be
recognized economically?
04
(c) Draw a neat diagram showing parts of biosensor; explain the
function of each of them.
07

Q.2 (a) How MATLAB is used for bioprocess data handling?
03
(b) Write a note on: microbial electrode for biosensor
04
(c) What is metastable region in crystallization? What does it
indicate? Explain the nucleation stage.
07
OR
(c) Explain the set up of electrodialysis. Write three application of it
in biotechnology.
07
Q.3 (a) What is SIMULINK?
03
(b) Write a note on: FIA
04
(c) Make a comparison of various downstream processes from
economy point of view.
07
OR
Q.3 (a) Discuss the principle of Mass spectrometry.
03
(b) Show just a tree view to exhibit the classification of models.
04
(c) Explain the media optimization technique and its importance.
07
Q.4 (a) Give the application of FTIR and GC-MS.
03
(b) What is selective extraction? Explain.
04
(c) The lab scale experimental data for the adsorption of an
antibiotic on activated carbon are as follows.
S(mg/cm
3
) Ca (mg/g)0.3 0.15
0.12 0.12
0.04 0.095
0.018 0.08
0.006 0.06
0.001 0.045
Find out to which Adsorption
isotherm, the data fit.
07

1
Seat No.: ________ Enrolment No.___________
GUJARAT TECHNOLOGICAL UNIVERSITY
BE – SEMESTER ?VIII (NEW) – EXAMINATION ? SUMMER 2018
Subject Code: 2180408 Date: 30/04/2018
Subject Name: Biochemical Engineering-II
Time: 10:30 AM to 01:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
4. Notations / abbreviations have conventional meaning and needs no clarification.

MARKS
Q.1 (a) What are the objectives of downstream processes?
03
(b) What are the categories of microbial product that can be
recognized economically?
04
(c) Draw a neat diagram showing parts of biosensor; explain the
function of each of them.
07

Q.2 (a) How MATLAB is used for bioprocess data handling?
03
(b) Write a note on: microbial electrode for biosensor
04
(c) What is metastable region in crystallization? What does it
indicate? Explain the nucleation stage.
07
OR
(c) Explain the set up of electrodialysis. Write three application of it
in biotechnology.
07
Q.3 (a) What is SIMULINK?
03
(b) Write a note on: FIA
04
(c) Make a comparison of various downstream processes from
economy point of view.
07
OR
Q.3 (a) Discuss the principle of Mass spectrometry.
03
(b) Show just a tree view to exhibit the classification of models.
04
(c) Explain the media optimization technique and its importance.
07
Q.4 (a) Give the application of FTIR and GC-MS.
03
(b) What is selective extraction? Explain.
04
(c) The lab scale experimental data for the adsorption of an
antibiotic on activated carbon are as follows.
S(mg/cm
3
) Ca (mg/g)0.3 0.15
0.12 0.12
0.04 0.095
0.018 0.08
0.006 0.06
0.001 0.045
Find out to which Adsorption
isotherm, the data fit.
07

2

OR
Q.4 (a) What principle govern the precipitation process?
03
(b) Discuss dissociative extraction.
04
(c) Adsorption

of an organic solute on activated silica gel gave the
following data after equilibrium.
S(mg/cm
3
) Ca (mg/g)0.139 0.03
0.089 0.026
0.066 0.0225
0.047 0.021
0.037 0.018
Fit the data to an adsorption isotherm and calculate rate constant.
07
Q.5 (a) Give the principal of sedimentation.
03
(b) Enlist the types of separator in centrifuge, with its specific use.
04
(c) Write a note on: High speed ball mills
07
OR
Q.5 (a) Compare and comment of disruption of bacterial and fungal cell.
03
(b) What is the significance of Schulze-Hardy rule?
04
(c) With the sketch, discuss the Ion exchange chromatography.
07
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